- https://www.sincrotroalba.cat/en/media/events/alba-public-events/cryo-electron-microscopy-in-quest-for-resolution-from-evolution-to-revolution
- Cryo-Electron Microscopy in quest for resolution: From Evolution to Revolution
- 2016-09-19T12:00:00+02:00
- 2016-09-19T13:00:00+02:00
- Prof. Jose L. Carrascosa. Department of Structure of Macromolecules. Centro Nacional de Biotecnologia, CSIC. Campus Cantoblanco. Madrid
- What
- events
- When
- Sep 19, 2016 from 12:00 PM to 01:00 PM (Europe/Madrid / UTC200)
- Where
- ALBA Synchrotron - Maxwell Auditorium
- Contact Name
- Inma Hernández
- Contact Phone
- +34 93 592 43 89
- Web
- Visit external website
- Add event to calendar
-
iCal
After a number of years of relatively slow improvement, recent advances in cryo–electron microscopy (cryo-EM) have finally allowed structures of large macromolecules and macromolecular complexes to be determined at near-atomic resolution. Achievement of resolutions below 4 Å are extending in very different types of macromolecules, with selected examples extending up to better than 2 Å for around 350 kD protein complexes. These results not only support the incorporation of cryo-EM as a technique of choice for structural determination, but also demonstrate that the density maps are detailed enough to show water molecules, ions, and precise side-chain conformations.
As the analysis of images provided by cryo-EM is done particle by particle, structural heterogeneity can be studied in detail, providing unprecedented insights about structural features involved in key steps of their function and regulation. The fact that cryo-EM allows the atomic resolution analysis of complexes rapidly frozen from solution, and thus under conditions closer to physiological conditions, offers a great potential of using cryo-EM and single-particle analysis to study the protein–ligand interactions and structure-based drug design, while opening new opportunities for the study of detailed protein functional mechanisms.
